Effect of chemical agents on nucleic acid and protein synthesis in rat tumour tissue in vivo.

Abstract

1. In an earlier paper it was shown that 2000 r of X radiation applied to the Jensen rat sarcoma in vivo reduced the uptake of 32P into the deoxyribonucleic acid fraction. X radiation did not reduce the uptake of 32P into the ribonucleic acid fraction, nor did it reduce the uptake of methionine into proteins. 2. It has now been shown that 2 : 4-dinitro-phenol, sodium pyrophosphate and adenyl pyrophosphate reduce the uptake of 32P into both nucleic acid fractions and reduce the uptake of methionine into the ribonucleo-protein and the heat-coagulable protein fractions. 3. The possible significance of these results for determining the primary action of X rays on nucleic acid synthesis is discussed.