Abstract
Female gametophytes of Laminaria angustata KJELLMAN formed oogonia in PESI medium but scarcely formed them in ASP12 NTA medium at 10°C, 14 h light-10 h dark cycle and 2, 000-3, 000 lx. The oogenesis eddects of nitrate, phosphate, iodine, boron, iron, manganese, zine and cobalt were investigated in ASP12 NTA as a basal medium. Under this condition, nitrate, phosphate, manganese, zinc and cobalt were not effective in oogonium formation. Iodine seemed to be slightly effective. Boron had a tendency to inhibit oogonium formation. However, iron strongly induced oogomiu, formation of L. angustata, and most female gametophytes formed oogonia at 2.0mg/l EDTA chelated iron after two weeks, at which time almost all cells of female gametophytes became eggs. These eggs developed into parthenogenetic sporophytes which showed abnormal morphology.
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