Effect of Chaiqinchengqi decoction on inositol requiring enzyme 1α in alveolar macrophages of dogs with acute necrotising pancreatitis induced by sodium taurocholate

Abstract

ObjectiveTo investigate the effect of Chaiqinchengqi decoction (CQCQD) on inositol requiring enzyme 1α (IRE1α) in alveolar macrophages (AMs) of the dog model of acute necrotising pancreatitis (ANP) induced by sodium taurocholate. MethodsFifteen beagle dogs were randomised into a control group, ANP group and CQCQD group (n = 5 per group). ANP was induced by a retrograde duct injection of 50 mg/kg of 5% sodium taurocholate. The dogs in the control group received injections of the same volume of saline as the sodium taurocholate. After the models were induced, the dogs in the CQCQD group were administered 10 mL/kg CQCQD every 2 h for 6 h. Two hours after the last administration of either CQCQD or saline, they were sacrificed by anaesthesia. AMs were collected to determine the IRE1α and Interleukin-1β (IL-1β) mRNA and protein expression, and pancreatic tissues were collected for histopathology analysis. ResultsCompared with the ANP group, the mRNA and protein expression of IRE1α and the protein expression of IL-1β of AMs in the CQCQD group were significantly down-regulated, and the pancreatic histopathology score of the CQCQD group also was lower. There was no significant difference in the mRNA expression of IL-1β of AMs between the two groups. ConclusionThe CQCQD-induced down-regulation of the IL-1β protein expression may involve the down-regulation of the mRNA and protein expression of IRE1α in AMs.