Abstract
Homologous oligosaccharides ranging from bioses to tetraoses were immobilized to 3-(β-aminoethoxy)propylsilica by reductive amination, and the chromatographic behaviour of concanavalin A (Con A) with the resultant gels was examined. Con A showed a strong affinity to immobilized mannose oligomsers having mainly α1 → 2 interglycosidic linkages, giving broadened peaks when phosphate buffers containing glucose as a displacing sugar were employed as eluents. The association constant ( K a) calculated from elution volume increased with increasing degree of polymerization, implying that not only the non-reducing monosaccharide residue but also the interior portion of the sugar chain took part in complexation. Con A showed a similar tendency to glucose oligomers linked through α1 → 6 bonds, although the K a values were much smaller than those of the corresponding mannose oligomers.
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