Abstract
ObjectivesTranscription of eukaryotic protein-coding genes by RNA polymerase II (pol II) is a highly regulated process. Most human genes have multiple poly(A) sites, which define different possible mRNA ends, suggesting the existence of mechanisms that regulate which poly(A) site is used. Poly(A) site selection may be mediated by cleavage factor I (CFIm), which is part of the cleavage and polyadenylation (CPA) complex. CFIm comprises CFIm25, CFIm59 and CFim68 subunits. It has been documented that the CPA complex also regulates pol II transcription at the start of genes. We therefore investigated whether CFIm, in addition to its role in poly(A) site selection, is involved in the regulation of pol II transcription.Data descriptionWe provide genome-wide data of the effect of reducing by 90% expression of the CFIm25 constituent of CFIm, which is involved in pre-mRNA cleavage and polyadenylation, on pol II transcription in human cells. We performed pol II ChIP-seq in the presence or absence of CFIm25 and with or without an inhibitor of the cyclin-dependent kinase (CDK)9, which regulates the entry of pol II into productive elongation.
Highlights
Data description: We provide genome-wide data of the effect of reducing by 90% expression of the CFIm25 constituent of cleavage factor I (CFIm), which is involved in pre-mRNA cleavage and polyadenylation, on pol RNA polymerase II (II) transcription in human cells
We performed polymerase II (pol II) chromatin immunoprecipitation (ChIP)-seq in the presence or absence of CFIm25 and with or without an inhibitor of the cyclindependent kinase (CDK)9, which regulates the entry of pol II into productive elongation
Proteins involved in pre-mRNA cleavage, such as the cleavage and polyadenylation specificity factor (CPSF) complex, regulate pol II activity at the beginning and end of the transcription cycle [12]
Summary
Data description: We provide genome-wide data of the effect of reducing by 90% expression of the CFIm25 constituent of CFIm, which is involved in pre-mRNA cleavage and polyadenylation, on pol II transcription in human cells. We performed pol II ChIP-seq in the presence or absence of CFIm25 and with or without an inhibitor of the cyclindependent kinase (CDK)9, which regulates the entry of pol II into productive elongation. Objective The production of a eukaryotic protein-coding mRNA requires the recognition of a specific poly(A) site sequence at the end of the gene.
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