Abstract
Staphylococcus aureus is one of the species that are most frequently isolated from medical devices. The ability to produce a biofilm is an important step in the pathogenesis of these staphylococci; biofilm formation is strongly dependent on the environmental conditions and also on the antibiotics and disinfectants used in the treatment of infections. In this study, 28 staphylococcus aureus isolated from medical devices at the University Hospital Center of Sidi Bel Abbes (in Northwestern Algeria) were analyzed to detect the formation of biofilm by culture on Red Congo Agar (RCA). The Tube Method (TM) and tissue Culture Plate (TCP) techniques were also used to investigate the effect of penicillin, ethanol and Betadine on the preformed biofilm. It has been found that 19 strains produced a bacterial slime on the Congo red medium, 7 strains produced a biofilm by the tube method, 2 of which are highly productive. In addition, 9 strains produced a biofilm on polystyrene micro-plates; this number was higher in the presence of penicillin and ethanol with 19 and 11 biofilm producing strains, respectively. On the other hand, no biofilm was formed in the presence of Betadine. It is important to examine the response of biofilms following an imposed external constraint such as disinfectants and antibiotics in order to develop new strategies to combat bacterial biofilms but also to better control their formation.
Highlights
Staphylococcus aureus is one of the major causes of nosocomial and community-acquired infections
The strains selected in this study were isolated from medical devices obtained from the Departments of Reanimation, Urology and Internal Medicine at the University Hospital Center (CHU) of Sidi Bel Abbes, a city located in northwestern Algeria
The results obtained by the Tube Method (Table 1) are different from those obtained by the Tissue Culture Plate (TCP) technique; only 7 strains produce biofilms by this technique, 2 of which are highly productive
Summary
Staphylococcus aureus is one of the major causes of nosocomial and community-acquired infections. This germ is responsible for acute and chronic infections; most of them are due to the ability of these germs to adhere to medical implants and form a biofilm (Verma and Singh, 2015). The biofilm is recognized as the most predominant form of development of bacteria in nature. These bacteria are complex communities of microorganisms, embedded in a self-secreted matrix of Extracellular Polymeric Substances (EPS) (Kara Terki et al, 2013). Infections associated with biofilms constitute a major clinical problem and are the cause of increased mortality; they generally require higher costs of medical treatment (Bellifa et al, 2016; Chessa et al, 2016; Badran et al, 2015)
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