Effect of cerium oxide nanoparticles preconditioning on rat myocardial cells oxidative damage

Abstract

Objective To study the effect of cerium oxide nanoparticles(Nanoceria) on myocardial cells oxidative damage and its possible mechanisms. Methods Myocardial cells culture in vitro were randomly divided into experimental group, model group and the blank group. The experimental group was added at a concentration of 10 nmol/L cerium oxide nanoparticles culture for 24 h, model group and the blank group with normal culture for 24 h, the final concentration of 25μmol/L H2 O2 were joined to experimental group and model group for 2 h, the blank group not treated.Detecting of total antioxidant capacity (T- AOC), superoxide dismutase(SOD), glutathione peroxidase(GSH- Px) and malondialdehyde (MDA)content in myocardial cells, nuclear factor related factor 2(Nrf2) distribution in cells and Nrf2, heme oxygenase- 1(HO- 1), nicotinamide adenine dinucleotide phosphate(NADPH):quinone oxidoreductase 1(NQO1) mRNA express. Results Compared with the model group,the experimental group SOD(92.05±7.39 vs.74.99±10.86), GSH- Px(15.76±2.75 vs.10.72±1.60) T- AOC,(1.15± 0.25 vs.0.59±0.11) content were significantly increased(P< 0.05),MDA(15.14±1.28 vs.18.48± 2.09)content decrease(P<0.05);With the blank group and the model group,the experimental group increased the Nrf2 nuclear translocation rate;Compared with the blank group and the model group,the experimental group Nrf2(1.700±0.270 vs.0.930±0.146, 0.610±0.102), HO- 1(2.200±0.282 vs. 1.030±0.109, 0.400±0.171), NQO1(1.920±0.161 vs.0.980±0.133, 0.490±0.257) mRNA expression were significantly increased(P<0.01). Conclusion (1) Nanoceria has a certain extent protective effect aganist oxidative damage in neonatal rat cardiomyocytes.(2) Nanoceria can induce the nuclear translocation of Nrf2 and upregulate genes Nrf2, HO- 1, NQO1 mRNA expression.(3)Nanoceria maythrough the Nrf2/antioxidant response element(ARE)signal pathway to protect neonatal rat cardiomyocytes from oxidative stress injury. Key words: Cerium oxide; H2 O2; Cardiomyocytes; Oxidative stress; Nuclear factor related factor 2/antioxidant response element signal pathway