Effect of Cellulase Genes Recombinant L. reuteri on Digestibility of Diet Crude Fiber and Adhesion in Gut of Chicken

Abstract

Background: The aim of this test was study the gut adhesion ability of recombinant Lactobacilli and their improvement of the digestibility of crude fiber in chick diet. Methods: The adhesion ability was observed through in vitro and in vivo tests. Intestinal epithelial cells and recombinant Lactobacillus were co-cultured for observation of adhesion. One hundred one-day-old chicks were randomly divided into four groups; C15, C73, C1573 and control. Each chick in C15, C73, C1573 and control were orally gavaged with 200 ìL of fermentation broth of recombinant L. reuteri XNY-Cel15, 200 ìL of fermentation broth of recombinant L. reuteri XNY-Cel73 and 200 ìL of broth containing equal XNY-Cel15 and XNY-Cel73, 200 ìL of MRS broth, once at the beginning of the trial respectively. Result: Recombinant L. reuteri adhered to chick intestinal epithelial cells which co-cultured together in vitro. After feeding for 30 days, the reporter genes of recombinant Lactobacillus was detected in the crop, small intestine and cecum of trial chicks, with their sequences corresponding to those of the insert genes. The digestibility of crude fiber in experimental groups was higher than that in control group and the digestibility of C15 was significantly higher than other groups (P less than 0.01). It showed that the recombinant Lactobacillus can survive in the digestive tract of chicken for more than 30 days and helped to decompose the crude fiber in the digestive tract.