Effect of cell plating density and extracellular matrix protein on cell growth of epithelial rests of Malassez in vitro.

Abstract

The present study investigated how cell plating density and extracellular matrix protein influence cell survival of epithelial rests of Malassez (ERM) in vitro. ERM cells were plated on culture dishes coated with laminin (LM), type IV collagen (type IV), or fibronectin (FN), or on noncoated dishes, (Non) at a cell density of 2 x 104-1 x 105/ml in a nonserum culture medium. XTT assays were performed to calculate the number of cells attached on the substrata after 6, 24, 48, and 72 h. Mean cell numbers were calculated, and significant differences were determined using ANOVA. When epithelial cells were cultured on various matrices at a cell density of less than 2 x 104/ml, the cells did not grow and then fell into apoptotic cell death, which was confirmed by transmission electron microscopy. The cell number was significantly increased in cells plated on FN compared to those on Non at a cell density of 4 x 104/ml-8 x 104/ml. These results suggest that both extracellular matrix proteins and cell-cell interactions may contribute to prevent apoptosis of ERM in vitro. Cell-cell interactions may be a more important factor than exogenous extracellular matrix proteins for the survival of ERM cells in vitro.