Effect of celecoxib and adriamycin on proliferation of breast cancer cell line MCF-7

Abstract

Objective To study the roles of celecoxib and adriamycin in the inhibition of proliferation and the induction of apoptosis human breast cancer cells MCF-7. Methods MCF-7 cells together with celecoxib or/and adriamycin of different concentration after fostering 24、48、72 hours were carried out, the inhibition of proliferation was measured by MTT assay. Cell cycle and apoptotic percentage were analyzed by flow cytometry(FCM). Results MCF-7 cells together with adriamycin (10mg/L) after fostering 24 hours were pressed down obviously(P<0.01), MCF-7 cells together with celecoxib(50μmol /L) after fostering 24 hours were pressed down obviously(P<0.01), the effect was more obviously in the group of celecoxib combined and ADM. The flow cytometry shows that the proportion of G0/G1 phase and the apoptotic rate wee incresed significantly in all groups, the apoptotic rate was significant between all groups and control group(P<0.01). Conclusions The inhibition of celecoxib and adriamycin against human breast cancer MCF-7 is obviously with time dependence and dose dependence. Celecoxib can induce the apoptosis of human breast cancer MCF-7. Anti-tumor action of celecoxib combined and adriamycin against human breast cancer is cooperated. Key words: Celecoxib; Adriamycin; MCF-7; MTT; Apoptosis