Effect of CD44 antibody on hyaluronic acid degradation caused by retinal Mueller cell

Abstract

Objective To observe the effect of CD44 antibody on the hyaluronic acid （HA） degradation mediated by retinal Mueller cell Methods Pig retinal Mueller cells from the posterior pole （2nd generation） were cultured in three different medium： without HA （group 1 ）, 0.01 mg/ml HA （group 2 ）. 10 μg/ml HA and CD44 antibody （group 3）. The cells in the group 2 and 3 were pre-cultured with HA and CD44 antibody, and the supernatant was collected. HA substrate gel electrophoresis was performed for HA degradation, while ELISA-Iike method was performed for HA binding protein. Results HA substrate gel electrophoresis showed white light double-band on blue background in groups 1 and 3, thicker double band or bright de-colored blocks in group 2. ELISA-Iike method showed that the absorb＇ance （A） value of groups 1, 2 and 3 were 0.310-1-0.025, 0.093±0.051, 0.025±0.069 respectively. The A value of group 2 was obviously lower than that of group 1 （t=28. 1, P〈0.01）, theA value of group 3 was significanlly higher than that of group 2 （t=26.9, P〈70.01）, but was the same as group 1 （t=4.92, P±0.05）. Conclusion CD44 antibody can inhibit the interaction between Mueller cells and HA, and thus reduce the HA degradation. Key words: Cell adhesion molecules/antagonists ＆ inhibitors; Hyaluronic acid/metabolism, Mueller cells