Effect of CD147 inhibitor tunicamycin and activator angiotensin II on gallbladder carcinoma cells

Abstract

Objective To research the effect of CD147 inhibitor tunicamycin and activator angiotensin Ⅱ (AngⅡ) on proliferation, invasion, and expression of CD147 and matrix metalloproteinase (MMP)-9 in GBC-SD cells. Methods GBC-SD cells were treated with different concentrations of tunicamycin (5, 10, 20 μl/ml), AngⅡ (5, 10, 20 μl/ml) alone or tunicamycin plus AngⅡ (tunicamycin 10 μl/ml + AngⅡ 20 μl/ml). The rate of proliferation was detected by cell counting kit-8 (CCK-8). The expression of CD147 and MMP-9 was detected by Western blotting. The ability of invasion was examined by Transwell assay. Results (1) As compared with the control group, proliferation rate of GBC-SD in tunicamycin group was significantly decreased in a time- and concentration-dependent manner [(88.17±8.36)% vs. (100.00±0.00)%, P=0.005; (85.35±6.42)% vs. (100.00±0.00)%, P=0.003; (70.15±6.89)% vs. (100.00±0.00)%, P=0.000]. As compared with the control group, the CD147 value of K was significantly decreased (0.75±0.04 vs. 0.88±0.07, P=0.046; 0.71±0.04 vs. 0.88±0.07, P=0.009), and MMP-9 value of K was also decreased (0.84±0.04 vs. 1.10±0.08, P=0.004; 0.67±0.10 vs. 1.10±0.08, P=0.000) in a concentration-dependent manner in in tunicamycin group. The invasive ability in tunicamycin group was significantly decreased as compared with control group (43.00±10.98 vs. 67.65±8.65, P=0.034; 32.46±7.71 vs. 67.65±8.65, P=0.005). (2) The cell proliferation rate in AngⅡ group was gradually increased in a time- and concentration-dependent manner [(104.32±5.05)% vs. (100.00±0.00)%, P=0.056; (108.25±6.57)% vs. (100.00±0.00)%, P=0.019; (115.50±8.65)% vs. (100.00±0.00)%, P=0.003], the CD147 value of K increased in a concentration-dependent manner (1.02±0.08 vs. 0.88±0.07, P=0.039; 1.08±0.05 vs. 0.88±0.07, P=0.005), MMP-9 value of K increased (1.28±0.06 vs. 1.10±0.08, P=0.034; 1.57±0.07 vs. 1.10±0.08, P=0.001), and the invasion ability increased (98.00±12.56 vs. 67.65±8.65, P=0.013) as compared with the control group. There was significant difference in cell invasion, CD147 expression and MMP-9 expression between the combined group and the control group. Conclusion Tunicamycin can inhibit the proliferation of gallbladder cancer cells, reduce the expression of CD147 and MMP-9, and AngⅡcan accelerate the proliferation of gallbladder cancer cells, and increase the expression of CD147 and MMP-9. The combined use of tunicamycin and AngⅡ can counteract the interaction. Key words: Gallbladder carcinoma cell; Tunicamycin; Angiotensin Ⅱ; CD147; Matrix metalloproteinase-9