Effect of caveolin-1 on differentiation of rat bone marrow messenchymal stem cells into neuron-like cells

Abstract

Objective To investigate the role of caveolin-1 in bone marrow mesenchymal stem cells(MSCs) differentiating into neuron-like cells by transfecting MSCs with small interfering RNA(siRNA). Methods Construct caveolin-1 siRNA and then transfect into MSCs cultured in vitro. Cell growth was observed by an inverted phase contrast microscope. The survival ratio of MSCs was determined by MTT before transfection,24 hour, 3 days after transfection,respectively. MSCs was induced to differentiate into neuron-like cells by-ME. The neural cell specific marker Nestin,NSE,GFAP were determined by immunocytochemistry stain technique. Results Expresion of caveolin-1 vanished by tranfection of caveolin-1 siRNA,survival ratio of MSCs by transfection decreased and there was significant differences between no-transfection and transfection-controlling group after 24 hours and 3 days of transfection(P<0.01).The differentiation efficiency of MSCs transfected by caveolin-1 siRNA into neuron-like cells and the expression of NSE were increased significantly than no-transfection and transfection-controlling group, The percentage of positive NSE was(75.5±2.5)% after 24 hours and(81.6±3.5)% after 6 days, there was no expression of GFAP. There was significant differences between no-transfection and transfection-controlling group(P<0.01). Conclusions The differentiation efficiency of MSCs increased by suppressing caveolin-1 expression. Key words: Marrow mesenchymal stem cells ; Neuron-like cells ; Caveolin-1 ; RNA interfering ;