Abstract

The effect of catalin on cross-linking in the DNA-protein system is studied after illumination with visible and UV light. Catalin is shown to stimulate this process. The use of a hydroxylamine trap reveals that catalin (10–100 μM) induces the accumulation of free radicals in aqueous solutions as soon as 10 minutes after irradiation. A considerable amount of O2- is fixed in the medium during illumination of catalin aqueous solution. Superoxide dismutase added to the incubation medium considerably reduced the number of recorded radicals.

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