Abstract
The oxidation of palmitate-1- 14C to 14CO 2 in homogenates of Ehrlich ascites tumor cells was increased approximately 2.5 times by the addition of carnitine. Carnitine palmityltransferase and long-chain fatty acid thiokinase activities were demonstrated in this homogenate. It is postulated that, as in non-enoplastic tissues, an acylcarnitine intermediate probably is involved in the oxidation of long-chain fatty acids by the tumor homogenate. Radioactive carnitine was taken up by the intact tumor cell, and a small fraction of this was incorporated into long-chain acylcarnitine. When exogenous palmitate was available, the incorporation of radioactive carnitine into long-chain acylcarnitine was doubled. However, exogenous palmitate-1- 14C oxidation by the intact tumor cell was not stimulated by addition of carnitine to the incubation medium, and deoxycarnitine did not inhibit palmitate oxidation by the intact cell. Added carnitine also had no effect on palmitate-1- 14C incorporation into the total lipid, phospholipid, triglyceride, or free fatty acid fractions of the intact cell.
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