Abstract
Background: Lung cancer is considered as one of the most common causes for cancer-related death globally. Carica papaya is one of the most well-known traditional medicines to treat diseases, and is also known to treat cancer and help in cancer prevention. The study investigates the effect of Carica seed extract on inhibitory kappa B kinase beta and mTOR mRNA expression in lung cancer cells (A549 cells).
 Methods: Cell viability test was assessed using MTT assay. mRNA expression of inhibitory kappa B kinase beta and mTOR mRNA was analyzed by real-time PCR. The results was analysed statistically by ANOVA and Duncan multiple range test with graphpad prism version 5 software. p<0.05 was considered significant.
 Results: It was observed that there wasmaximum inhibition (50%) at 400-500µg/ml of Carica seed extract. It was also found that the fold change over control of mTOR mRNA expression was significant at 500µg/ml of Carica seed extract and the fold change over control of IKKB mRNA expression was significant (p<0.05) at 400µg/ml in cancer cells treated with Carica seed extract.
 Conclusion: Thus concluding that Carica seed extract has been found to have significant anticancer property on A549 lung cancer cell lines, and can be used as a natural product in combating lung cancer.
Highlights
Cancer is one among the foremost deadly diseases of this century
Carica papaya extract significantly (p
400 to 500 μg/ml concentration of the extract showed maximum inhibition of the viability of the lung cancer cells suggesting that C. papaya has cytotoxic effect in A549 cells, as shown in (Fig. 1)
Summary
Cancer is one among the foremost deadly diseases of this century. Cancer is a life aggressive metabolic disease having a high mortality rate and the incidence rate is intensifying year by year. Insensitivity to growth-inhibitory signals, apoptosis evasion, induction of angiogenesis activation, and invasion leading to metastasis are some of the key characteristics of cancer [2]. These characteristics have prompted us to concentrate on pharmaceutical therapies aimed at inhibiting tumour development and progression by targeting inflammatory pathways to inhibit growth of tumour and its progression. The study investigates the effect of Carica seed extract on inhibitory kappa B kinase beta and mTOR mRNA expression in lung cancer cells (A549 cells). It was found that the fold change over control of mTOR mRNA expression was significant at 500μg/ml of Carica seed extract and the fold change over control of IKKB mRNA
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