Effect of cardioplegia solution of autologous blood treated with ultraviolet irradiation and oxygenation on myocardial mitochondrion in mongrel dogs underwent open heart surgery under cardiopulmonary bypass

Abstract

Objective To investigate the effect of cardioplegia solution of autologous blood treated with ultraviolet irradiation and oxygenation (UBIO) on myocardial mitochondrion in mongrel dogs underwent open heart surgery under cardiopulmonary bypass (CPB). Methods Twenty male mongrel dogs were randomly divided into a control group and a UBIO group, 10 dogs in each group. The UBIO group was infused with UBIO blood as the cardiac arresting solution via the ascending aorta, while the control group was given the same treatment except that the cardiac arresting solution was blood cardioplegia. Blood samples were taken from coronary venous sinus before cross-clamping and after aorta declamping to measure the levels of cardiac troponin I (cTnI) and creatinine kinase MB isozyme (CK-MB). For both groups, right atrial myocardial tissue samples were taken to detect the activity of myocardial mitochondrion superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX), and concentrations of malondialdehyde (MDA) when the right atrium was opened and closed. Results In both groups, the average levels of SOD and GSH-PX were decreased during CPB, and the decrease of SOD and GSH-PX were to a significantly larger extent in UBIO group (P<0.05). The concentrations of cTnI, CK-MB, and MDA in the control group were increased significantly higher than those in the UBIO group (P<0.05). Conclusion The attenuated lipid peroxidation of myocardial mitochondria plays an important role in myocardial protection by UBIO as cardioplegia solution in open heart surgery under CPB. Key words: Cardiopulmonary bypass; Ultraviolet blood irradiation and oxygenation; Myocardial protection; Mitochondrion