Effect of carbon nano colloid and its synergism with some antibiotics on Klebsiella pneumonia isolated from UTI of pregnant women

Abstract

Recently, Klebsiella pneumoniae has become a serious pathogen due to the serious diseases it causes including urinary tract infections especially in pregnant women pneumonia, liver abscess and gastrointestinal. Recent research has tried to find new ways to confront the resistance of these bacteria against different types of antibiotics. Out of 150 isolates that were isolated from urinary tract infections for pregnant women 24 were Klebsiella spp. Molecularly, K. pneumoniae isolates were identified as 16SrRNA of Klebsiella pneumoniae by using polymerase chain reaction. Carbon nano colloid was prepared to investigate the effect of its synergy with antibiotics on the growth of Klebsiella pneumoniae, resistant to a number of antibiotics. The results showed that the highest percentage of the number of bacterial isolates was in the age group 26-30 years. Molecular diagnostics showed that 17 of K. pneumoniae isolates gave a molecular size 274 base pair when PCR was performed using the primer targeting 16SrRNA. Two strains of Klebsiella pneumonia were selected as the most resistant isolates against antibiotics. Multiple concentrations of carbon nanoparticles were synergized with antibiotics and the results showed a broad effect in inhibiting the growth of bacterial isolates. Higher is the concentration of nanoparticles, higher is the sensitivity of Klebsiella pneumonia. The results recorded highest inhibition zoon of 35 mm in Kl.1 isolate and 34 mm in Kl.2 isolate at concentration (1:4) of carbon nanoparticles and Ceftriaxon antibiotic. The concentration of (1:4) of amikacin antibiotic and carbon nanoparticles recorded inhibition zoon (40 and 38 mm) of Kl.1 and Kl.2. The results of the synergistic effect of the antibiotic Gentamicin with carbon nanoparticles showing inhibition zoon diameters were 39 and 28 of the two isolates Kl.1 and Kl.2 respectively.