Abstract
Biological herbicides will assume greater importance for weed control as the number of chemicals registered for use decreases. Two promising fungi pathogenic on Datura stramonium and Cassia obtusifolia are, respectively, Alternaria crassa and Alternaria cassiae. In order to develop technologies for the efficient production of spore inocula in submerged liquid culture, we studied the effect of volatile factors which influenced sporulation on solid media. Sporulation was quantified on Richard's V8 agar under individual and joint variation of carbon dioxide (CO2) and relative humidity (RH). Sporulation of both fungi was suppressed when the two factors increased but not when CO2 was absorbed and RH remained high. Incubation of cultures in a CO2 controlled cabinet with RH constant indicated that CO2 was the suppressant.
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