Effect of carbon dioxide and relative humidity on self incompatibility in cauliflower, Brassica oleracea

Abstract

The events of the progamic phase of fertilization have been monitored by in vitro experiments in self compatible (SC), partial self-incompatible (PSI) and self incompatible (SI) lines. The duration of the progamic phase is about 30 h. Treatment with low concentrations of CO2 (3 to 5%) at high relative humidity (rH, 100%) had the following effects: pollen quality, which declines normally during flower ageing, was prematurely reduced; pollen adhesion and germination, both low in SI matings, were increased; the stigma callose response in SI matings was reduced to the low level of SC matings; and the number of pollen tubes in the style after SI matings significantly increased. CO2 concentrations of 4 to 6% applied for 8, 16 or 24 h at 100% rH proved to be the most effective treatment for blocking the SI response in cauliflower.