Effect of CAO released from MS-CAO on human hepatoma cell line SMMC-7721

Abstract

AIM:To explore the inhibitory effects and mechanisms of CAO released from CAO-MS on the growth of human hepatoma cell line SMMC-7721. METHODS:Inhibitory effects of CAO and CAO-MS on the growth of human hepatoma cell line SMMC-7721 in vitro were determined with MTT assay. The cell cycles of SMMC7721 treated with CAO and CAO-MS were analyzed with flow cytometry. The apoptosis of human hepatoma cell line was tested with cellular DNA quantitative analysis, Annexin V labeling and morphological study. The expressions of p21WAF1/CIP1 protein and mRNA were detected, respectively,with immunocytochemistry staining (ICC) and reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS:The inhibitory effects of CAO and CAO-MS on the growth of human hepatoma cell line were doseand timedependent (P <0.01). The IC50s of CAO and CAO-MS on SMMC-7721 were 50 mg/L and 100 mg/L, respectively. The propoertion of SMMC-7721 in G0/G1 phase increased and that in S and G2+M phase decreased after exposure with 50mg/L CAO and 100mg/L CAO-MS for 72 hours. ICC and RT-PCR analysis showed that both CAO and CAO-MS incresaed the level of p21WAF1/CIP1 protein and mRNA. The effect of CAO was superior to that of CAO-MS with the same dosage of CAO and CAO-MS. CONCLUSION:Dose-dependent inhibitory effects of CAO and CAO-MS on the growth of human hepatoma cell line are remarkable. Both CAO and CAO-MS could induce apoptosis of human hepatoma cell and keep the cell cycle at G0/G1 phase from up-regulating expression of p21WAF1/CIP1 protein and mRNA. Wu WY,Guo WJ,Chang G. Effect of CAO released from MS-CAO on human hepatoma cell line SMMC-7721. Shijie Huaren Xiaohua Zazhi 2003;11 (3):260-263