Abstract
Cannabinoids interact with membrane receptors causing, among others, psychoactive and motor effects. Recently it has been shown the presence of CB1 receptors in amphibian skeletal muscle. The aim of our study was to approach the role of acetylcholine receptors (AchR) on the mechanism of cannabinoid action in skeletal muscle contraction. We isolated bundles of the slow portion of cruralis muscle of the frog and induced contractures by incubating with Ringer solution with choline 115 mM. Choline induces maintained contractures with a slow relaxation phase.
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