Abstract
The present study investigated the effects of exogenous cyclic AMP (cAMP)-dependent protein kinase inhibitor (H89) on kinematic parameters of epididymal ram sperm in vitro. After incubation of epididymal sperm samples for 45 and 90 min in the presence of H89 (0.5, 5, and 50 μM), motion parameters were evaluated by computer-aided sperm analysis. At 50 μM concentration of H89, there was a significant decrease in the fast progressive motility (at 45 and 90 min), and also most of the kinematical parameters such as curvilinear velocity, straight line velocity, average path velocity, mean angular degree (MAD), amplitude of lateral head displacement, beat cross-frequency, linearity, and straightness but not in wobble (WOB) at 45 min. After 90 min all kinematic parameters were significantly decreased at 50 μM concentration except MAD and WOB. In conclusion, high concentrations of exogenous cAMP-dependent protein kinase inhibitor (H89) decrease the motion parameters of ram epididymal sperm.
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