Effect of calorific intake on proteomic composition of colostrum in dairy cows

Abstract

The amount of concentrated feed supplied to a dairy cow affects milk yield. However, there is no evidence of a relationship between the colostrum proteomic composition and energy intake. We supplied 30 heifers (4–24 months old, two groups of 15 heifers each) with either a normal diet and high-energy diet to investigate the correlation between energy intake and colostrum protein composition. Colostrum milk proteins were analysed on the day of calving and on the third day following calving using two-dimensional gel electrophoresis (2-DE) and peptide mass fingerprinting (PMF). Five proteins were identified as differentially expressed between the two feeding groups in the colostrum on the day of calving. The levels of αS2-casein precursor and β-casein was higher in the colostrum from the high-energy diet group (HEG), whereas the levels of IgG3 heavy chain constant region, non-classical MHC class I antigen isoform X2, and β-casein A2 variant were higher in the normal-diet group (NEG) colostrum. Twelve differential proteins were identified on the third day: β-lactoglobulin, αS2-casein, zinc-α2-glycoprotein, lactoferrin, fibrinogen gamma-B chain isoform X1, non-classical MHC class I antigen isoform X2, complement C3, gelsolin isoform A precursor, vitamin D-binding protein isoform X1, immunoglobulin gamma 1 heavy chain constant region, IgG3 heavy chain constant region and polymeric immunoglobulin receptor. All were present at higher levels in the normal-diet group colostrum than in the high-energy diet group colostrum, although the milk yield from mature cows was lower in the normal-diet group. In conclusion, a high-energy diet can enhance milk production; however, the levels of immune-related factors are higher in the colostrum of cows fed a normal diet.