Abstract
Publisher Summary This chapter discusses the the effect of calmodulin and proteolytic digestion of the membrane on the active calcium transport and membrane phosphorylation. Active calcium transport is a basic function of the mammalian plasma membranes and the human red cell membrane is a suitable system for studying the characteristics of this calcium pumping. Calcium extrusion from red cells requires intracellular ATP and magnesium and the active centre of the pump is at the internal membrane surface. The preparation of inside-out red cell membrane vesicles (IOV) provides the possibility of adjusting ATP, magnesium, and calcium concentrations at the active centre by changing the concentrations in the bathing medium. Calcium transport, CaMg-ATPase activity, and calcium-induced membrane phosphorylation are studied simultaneously in this preparation. IOVs accomplish an ATP + Mg-dependent uphill calcium uptake, and this process is significantly stimulated by calmodulin, a cytoplasmic regulatory protein of calcium-dependent processes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
