Effect of Calcium Sulfide Nanoclusters in the Migration of Non‐Small Cell Lung Adenocarcinoma cell line HCC827

Abstract

Lung cancer is the most common malignancy worldwide independently of gender. Non‐small cell lung carcinoma (NSCLC) is responsible for 80% of diagnosed cases. Prognosis for patients suffering this cancer is poor, with only 7% of patients surviving five years after the diagnosis. Current treatments include surgery, radiotherapy, and chemotherapy. Although the aforementioned seem successful, they do not address the development of secondary tumors due to cell migration resulting in metastasis. Evidently, there is a need for developing novel therapeutic approaches specifically targeting malignant cells. In our laboratory, we evaluate calcium sulfide (CaS) nanostructures as potential multi‐cancer malignant‐cell targeted specific treatment. The objective of this study was to determine the effect of CaS nanoclusters in the migration of NSCLC HCC827 cells. We hypothesized that CaS will decrease the area of migration compared to the control vehicle (DMSO), will increase the number of dead cells and decrease the live cells in the collected supernatant. We used the Scratch Wound Healing Assay, to determine the area of migration after 24, 48, and 72hrs upon a single exposure at time 0 of CaS treatment and controls (DMSO: vehicle control; etoposide: positive control; and media: negative control). We also collected the supernatant to characterize detached cells in suspension. Preliminary data shows that at 72hrs, cells treated with CaS exhibited a significant decrease in cell migration. We categorized the cells in the supernatant as live or dead. Although there were not statistically significant differences when compared CaS treatment with the controls, we observed a time‐dependent trend. This trend showed a decrease of the number of dead cells and an increase of live cells as the time increases. Based on this preliminary data, we partly accept our hypothesis due to the observed decreasing of migration in CaS treatment. However, the supernatant data was the opposite to what we hypothesized. We are currently optimizing experiments to increase statistical power and correlate the area of migration with the supernatant findings. In the next step of this project, I will be determining the effect of CaS by means of migration and number of live and dead cells in the supernatant in the non‐malignant lung cells MRC5.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.