Abstract
A disaggregated cell system prepared from rat renal cortices has been developed to study the regulation of renin release in vitro. Cell suspensions were prepared by incubating minced renal cortical tissue in collagenase. The digested tissue was filtered sequentially through graded Teflon meshes of 125 and 44 mu, respectively. This preparation contained less than 2% intact glomeruli or tubules. Incubation of cell suspensions with the beta-adrenergic agonist L-isoproterenol (ISO) significantly increased the activity of renin released into the incubation media. Peak levels of renin activity were detected 10 to 15 min after the addition of ISO. The apparent ED50 for stimulation of renin release by ISO was 6 X 10(-8)M and the response was antagonized by the beta-adrenergic antagonist dl-propranolol. The spontaneous release of renin was also suppressed by increasing the concentration of extracellular calcium, whereas sodium ions had no effect on this process. These data have demonstrated that disaggregated renal cortical cell models are useful for studying the in vitro effects of pharmacologic agents and ions on renin secretion.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
