Effect of calcium lactate on m-calpain activity and protein degradation under oxidising conditions

Abstract

In two experiments, the effects of calcium lactate (CAL) on calpain activity were determined. In the model system, purified porcine skeletal muscle m-calpain was pre-incubated with various combinations of hydrogen peroxide (H2O2), calcium chloride, and/or different CAL concentrations. The m-calpain was activated by CAL, and the extent of m-calpain oxidation by H2O2 was significantly decreased with increasing CAL concentrations. In the muscle system, a beef longissimus lumborum (1day postmortem) from each carcass (n=6) was cut in half, randomly assigned to either 0.2M CAL or water injection (WAT), and then packaged in a high-oxygen modified atmosphere. The CAL injected steaks resulted in less intact desmin and greater production of a 30-kDa troponin-T compared to the steaks in the WAT group. The CAL injection did not affect colour and lipid oxidation of steaks during display. These results suggest that CAL addition may improve tenderness of meat by enhancing activation of endogenous calpain and by protecting against calpain oxidation under oxidative conditions.