Effect of calcium ion on the morphology structure and compression elasticity of muscle fibers from honeybee abdomen

Abstract

Muscle contraction activated by calcium ion is the key to reveal that honeybee abdomen can achieve various physiological activities through flexible exercises and contributes to a powerful mechanical function of muscle fibers. To investigate the stimulating effect of calcium ion on muscle fibers of honeybee abdomen, atomic force microscopy was used to measure the morphology structure and mechanical properties of muscle fibers from honeybee abdomen in different calcium ion solutions. The periodic morphology structure of muscle fibers stimulated by different calcium ion concentration changed greatly, and the sarcomere length contracted from 6.53 μm to 4.29 μm as the calcium ion concentration increased from 0.11 mM to 10 mM. The mechanical measurement showed that the elastic modulus of Z-line reached the maximum, followed by M-line, overlap zone and I-band in sequence at the same calcium ion concentration, and was approximately 3.636, 2.450, 2.284, 2.748 times that of I-band from 0.11 mM to 10 mM calcium ion concentration. Combining the experimental analysis, the calcium ion threshold range was obtained based on the response surface method. This work adequately elucidates biological structure and biomechanics of muscle fibers from honeybee abdomen and could provide reference for other similar muscle system.