Effect of Calcium-enriched Mixture (CEM) cement on increasing mineralization in stem cells from the dental pulps of human exfoliated deciduous teeth.

Rezvan Rafatjou,Atousa Janeshin,Iraj Amiri

doi:10.15171/jpid.2018.036

Abstract

Background. Stem cells isolated from human exfoliated deciduous teeth (SHED) are highly capable of proliferation and differentiation into odontogenic, osteogenic, adipose tissue and neural cells. The aim of this study was to investigate the effect of CEM cement on increasing mineralization in stem cells of exfoliated deciduous teeth. Methods. Dental pulps were isolated from extracted exfoliating primary teeth and immersed in a digestive solution. The dental pulp cells were immersed in α-MEM (modified culture medium) and 10% fetal bovine serum (FBS) was added. The culture cells were used for mineral deposit formation after the third passage. The cells were cultured in osteogenic cell culture medium in the control group and in osteogenic culture medium supplemented with CEM cement in the case group. Alizarin red staining was used to evaluate the mineral deposit formation on day 21. Statistical significance was determined with t-test. Results. Quantification of alizarin red staining showed that cells exposed to CEM cement induced more mineralized nodules (P=0.03). Conclusion. Mineral deposit formation in SHEDs was stimulated by CEM cement. Based on these data it might be suggested that CEM could improve osteoblastic differentiation.

Highlights

During tooth development, interactions between epithelial and mesenchymal cells lead to differentiation of odonotoblasts which deposit specialized mineralized dentin.[1]
Mineral deposit formation in stem cells from human exfoliated deciduous teeth (SHEDs) was stimulated by Calcium-enriched mixture (CEM) cement
Based on these data it might be suggested that CEM could improve osteoblastic differentiation

Summary

Introduction

Interactions between epithelial and mesenchymal cells lead to differentiation of odonotoblasts which deposit specialized mineralized dentin.[1]. Mesenchymal stem cells (MSCs) isolated from various adult tissues are common candidates for tissue engineering These cells are highly proliferative and multipotent with the capability to differentiate into several cell types; they have been considered as a potential cell source for bone regenerative therapies.[4,5] Dental pulp stem cells (DPSCs) are considered a good source of multipotent mesenchymal stem cells and have the ability to differentiate into odontoblasts, osteoblasts, chondrocytes, myocytes and adipocytes.[6] Dental stem cells can be isolated from several dental sources, including DPSCs,[3] stem cells from human exfoliated deciduous teeth (SHEDs),[7] periodontal ligament stem cells (PDLSCs),[8] stem cells from the apical papilla (SCAPs)[9] and dental follicle precursor cells (DFSCs).[10]. The aim of this study was to investigate the effect of CEM cement on increasing mineralization in stem cells of exfoliated deciduous teeth

Objectives

Methods

Results

Discussion

Conclusion