Abstract
Publisher Summary This chapter discusses a study to investigate the effect of calcium and nickel ions on contractile activity and metabolism of uterine smooth muscle isolated from 20-day pregnant rats. A further aim of the study was to analyze glycolytic and oxidative metabolic changes related to contractile energy dissipation and active ion transport. Longitudinal strips of rat uterus were suspended isomerically and tension development was measured. Tissue lactate production was estimated by determining changes in medium lactate concentrations. The spontaneous mechanical activity of uterine strips decreased continuously in Ca-free medium, which was accompanied by an increase of tissue NADH fluorescence intensity. Experimental results show that metabolic changes because of the activation of ion transport and contractile activity can be separately studied in the uterus. Tissue NADH fluorescence and lactate production measurements revealed that in this smooth muscle membrane, ion transport affects primarily glycolysis, while contractile energy dissipation activates mitochondrial respiration.
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