Abstract
Buffalo bull semen samples were diluted (1:40) in Cornell University extender, Cornell University-16 extender, egg yolk citrate, citric acid whey, lactose and tris diluents. These were frozen in liquid nitrogen (−196°C), thawed at 50°C and the effect of 6mM caffeine citrate on the postthawed motility was evaluated. Caffeine citrate increased the postthawed motility of the spermatozoa in different diluents from 25% to 100%. Highly significant stimulation due to caffeine citrate was observed in samples having low postthawed motility (upto 39%) irrespective of the diluents used.
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