Effect of Cadmium Ions on Some Biophysical Parameters and Ultrastructure of Ankistrodesmus sp. В-11 Cells

Abstract

Effects of low concentrations of cadmium ions on growth, photosynthesis, and cell ultrastructure of microalga Ankistrodesmus sp. B-11 were investigated. The addition of cadmium to growth medium at concentrations of 0.005–0.02 mg/L led to a significant decrease in the number of Ankistrodesmus sp. B-11 cells relatively to that in the untreated culture. The addition of cadmium at concentrations >0.05 mg/L completely stopped cell growth. Cadmium ions induced ultrastructural changes in the arrangement of thylakoids within the stroma, the detachment of thylakoid membranes with the formation of void interthylakoid spaces, and a significant increase in vacuolization of microalgal cells. Simultaneous measurements of fluorescence induction curves and redox transformations of photosystem I components on a microsecond time scale by means of a M‑PEA-2 fluorometer revealed that cadmium ions inhibit electron transport in photosystem II (PSII). The quantum yield of electron transport in PSII (φEo) and the performance index (PIABS) were found to decrease; the photoreduction of P700 pigment was decelerated, while energy dissipation (DI0/RC) and ΔpH-dependent nonphotochemical quenching (qE) increased significantly under the action of cadmium. The performance index (PIABS) was the most sensitive parameter; it can be used for the detection of early toxic effects of cadmium ions on algae.