Effect of C60 Fullerene on Recovery of Muscle Soleus in Rats after Atrophy Induced by Achillotenotomy.

Dmytro Nozdrenko,Kateryna Bogutska,Natalia Y Nurishchenko,Olexandr Motuziuk,Olga Abramchuk,Yuriy Prylutskyy,Peter Scharff,Svitlana Prylutska,Uwe Ritter

doi:10.3390/life12030332

Abstract

Biomechanical and biochemical changes in the muscle soleus of rats during imitation of hind limbs unuse were studied in the model of the Achilles tendon rupture (Achillotenotomy). Oral administration of water-soluble C60 fullerene at a dose of 1 mg/kg was used as a therapeutic agent throughout the experiment. Changes in the force of contraction and the integrated power of the muscle, the time to reach the maximum force response, the mechanics of fatigue processes development, in particular, the transition from dentate to smooth tetanus, as well as the levels of pro- and antioxidant balance in the blood of rats on days 15, 30 and 45 after injury were described. The obtained results indicate a promising prospect for C60 fullerene use as a powerful antioxidant for reducing and correcting pathological conditions of the muscular system arising from skeletal muscle atrophy.

Highlights

Functional unloading of mammalian skeletal muscles caused by partial immobilization can cause their atrophy
Regular strength training significantly increases the intensity of protein synthesis and, as a result, leads to hypertrophy of muscle fibers [3]
Functional unloading leads to suppression of protein synthesis and activation of proteolysis, which is reflected in a decrease in the diameter of muscle fibers and loss of their strength of contraction [4]

Summary

Introduction

Functional unloading of mammalian skeletal muscles caused by partial immobilization can cause their atrophy. In this case, the deepest atrophic changes are observed in the muscle soleus—the key postural muscle [1]. Functional unloading leads to suppression of protein synthesis and activation of proteolysis, which is reflected in a decrease in the diameter of muscle fibers (atrophy) and loss of their strength of contraction [4]. Muscle atrophy caused by prolonged inactivity is associated with both suppression of the intensity of protein synthesis and activation of intracellular proteolysis systems, which has been found in numerous animals and human model studies [5]. It has been shown that even short periods (5 days) of unuse of muscles can cause a significant loss of their mass and strength of contraction as well as accompaniment of physiological molecular rearrangements [6]

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Conclusion