Effect of Butylated Hydroxyanisole on Conidial Germination of Toxigenic Species of Aspergillus flavus and Aspergillus parasiticus

Abstract

The fungitoxic effect of butylated hydroxyanisole (BHA) on germination of conidia from seven toxigenic strains of Aspergillus was examined on potato dextrose agar (PDA). At concentrations of 200 μg/ml and above, BHA prevented germination of conidia in all of the test fungi after 24 and 48 h of incubation. On the other hand, at 100 μg/ml BHA reduced the rate of germination. When an impregnated disk assay was employed, distinct inhibition zones were observed at 200, 400, 800, and 1000 μg/disk of BHA after 24 h of incubation. After 48 h of incubation, distinct inhibition zones were still observed at 800 and 1000 μg/disk.