Abstract
Bursa of Fabricius tissues of untreated four-week-old and of cyclophosphamide-treated 10-day-old chickens were cultured by a perfusion method and the perfusate used in a study on the enhancement of antibody production and conversion in chickens. The bursal perfusates were given to incompletely immunosuppressed chickens (surgical bursectomy or hormonal bursectomy) and to control chickens which were immunised with either sheep red blood cells or Brucella abortus. The results indicated that bursal perfusate was successful in accelerating the IgM production in surgically bursectomised chickens and in stimulating IgG production. We were able to stimulate IgG production in IgM producing hormonally bursectomised chickens and to shorten the latent period of antibody production against B abortus antigen in normal chickens.
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