Abstract
Objective To evaluate the effect of bupivacaine on miniature excitatory postsynaptic currents (mEPSCs) and miniature inhibitory postsynaptic currents (mIPSCs) of neurons in hippocampal CA1 region of rats. Methods Twenty SPF healthy male Sprague-Dawley rats, aged 4-6 weeks, weighing 300-350 g, were divided into control group (C group, n=10) and bupivacaine model group (BPV group, n=10) using a random number table method.The central nervous system toxicity model was established by infusing 0.75% bupivacaine at a rate of 1 mg·kg-1·min-1 via the tail vein in BPV group, while normal saline was infused for 7.5 min at a rate of 1 mg·kg-1·min-1 via the tail vein in C group.Rats were sacrificed at 6 h after successful establishment of the model, the brains was removed and hippocampal slices were prepared.The whole-cell patch-clamp technique was used to record the frequency and amplitude of mEPSCs and mIPSCs. Results Compared with C group, the frequency of mIPSCs was significantly decreased (P 0.05). Conclusion The mechanism of bupivacaine-induced central nervous system toxicity is related to decreasing the frequency of mIPSCs in hippocampal neurons of rats. Key words: Bupivacaine; Drug toxicity; Central nervous system; Hippocampus; Neurons; Electrophysiology; Synaptic transmission
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