Effect Of Branched-chain Amino Acid Plus Glucose Supplement Timing On DOMS And Related Indicators After Eccentric Exercise

Abstract

PURPOSE: Nutrient timing is a strategic approach to maximize training effects, reduce risk of injury, and help with recovery. The present study examined the effect of BCAA plus Glucose on markers of muscle damage and Inflammation after eccentric exercise in male college students. METHODS: 18 healthy college students were divided into control group (PLA) group and supplement (BCAA+G) group randomly. Each group was randomly assigned 4 people for pre-exercise supplementation and 4 people after exercise for the first time, and changed for the second time. Before or after supplementation, volunteers performed an eccentric exercise protocol. Muscle soreness(VAS), creatine kinase (CK), C-reactive protein (CRP) and interleukin-6 (IL-6) and 3-methylhistamine (3MH) assessments were performed before exercise and after 30min, 24, 48 hours. RESULTS: The VAS score of the subjects increased significantly 24 hours after high-intensity eccentric exercise(2.86 vs 1.66, p<.05), and the increase of serum CK level(364.45 vs. 151.02U/L ), CRP(4.77 vs. 3.28mg/L) and IL-6(279.00 vs. 110.63pg/ml) increased significantly(p<.05); BCAA plus G supplement significantly reduced the VAS score(1.27 vs 2.86, p<.05) and CK(258.74 vs. 364.45U/L), CRP(3.75 vs. 4.77mg/L), IL-6(164.09 vs.279.00pg/ml) levels(p<.05), compared with pre-supplementation group, post-supplementation had lower VAS score(1.27 vs. 2.63), CRP(3.75 vs. 4.26mg/L) and IL-6(164.09 vs. 226.66pg/ml) and 3MH(105.07 vs. 131.67umol/L) response at 24 h after eccentric exercise(p<.05). CONCLUSIONS: High-intensity eccentric exercise caused DOMS with the elevation of damage and inflammatory markers as CK, CRP and IL-6. BCAA plus G supplementation can effectively reduce the level of DOMS, decrease muscle damage and inflammatory factors and protein breakdown. Compared with pre-exercise supplementation, post-exercise supplementation has a better effect on reducing inflammatory factors and protein breakdown caused by DOMS.