Effect of Brain-Cor Injection of Ginkgo on the Hyperlipemia and Thrombosis: Fundamental Study

Abstract

We have done this study to verify effects on the serum lipid content (TC, TG, HDL-C) in experimental model of hyperlipemia and on the blood coagulability in experimental model of thrombosis. Background: Female hormone preparation is limited in long term use because of hyperlipemia and thrombosis that are complications occurring in long term use of female hormone preparations for dysfunctional uterine bleeding, climacteric disorder, female genital organs tumor. Therefore to prevent complications such as hyperlipemia and thrombosis with female hormone preparation use are the acute problems not only for the effective treatment of gynecological diseases but also for the lowering of incidence rate of hypertension, arteriosclerosis, hyperlipemia and thrombosis. We made brain-cor injection of Ginkgo for the treatment of hyperlipemia and thrombosis that are complications occurring in long term use of female hormone preparations and verified its effects on the serum lipid content in experimental model of hyperlipemia and on the blood coagulability in experimental model of thrombosis. Objects and Materials: Wistar rats with 160~180 g in body weight were used as experimental animals, brain-cor injection of Ginkgo(70mg/20mL) made from Ginkgo extract that consists of flavonoid content above 24%, total lactone content above 6% and total ginkgolic acid content below 5μg/g was used as drugs. Methods: For 15 days brain-cor injection of Ginkgo 10mg/kg was injected in abdominal cavity of experimental model of hyperlipemia by tween-80, serum was separated from collected blood in orvita vein, serum TC (Total Cholesterol) content, serum TG (Triglyceride) content and serum HDL-C (High Density Lipoprotein-Cholesterol) content were examined with blood automatic biochemical analyzer and measurements were compared each other. For 15 days brain-cor injection of Ginkgo 10mg/kg was injected in abdominal cavity of experimental model of hyperlipemia and thromboplastin 0.5% 0.1mL/10g was injected on cauda vein in an hour after the last injection of Ginkgo. BT (Bleeding Time), CT (Clotting Time), APTT (Activated Partial Thromboplastin Time) and PT (Prothrombin Time) were examined and measurements were compared each other. Results: Brain-cor injection of Ginkgo decreased serum TC and TG content and increased serum HDL-C content in experimental model of hyperlipemia. And brain-cor injection of Ginkgo prolonged BT, CT, APTT and PT significantly in experimental model of thrombosis.