Abstract
The dissociation constants for binding of ADP at the adenine nucleotide (AdN) translocation site in heart mitochondria are decreased approximately 100-fold by bongkrekic acid (BA), indicating a strong increase in the affinity of the carrier for AdN. Accordingly, the rate of dissociation of AdN from the carrier sites is greatly decreased and becomes rate-limiting in the AdN translocation. The application of BA before or after equilibration of the endogenous pool with 14C-ADP also permits evaluation of the proportion of carrier sites (25%) accessible only to the endogenous pool.
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