Effect of bone regeneration of CMC- and HA-based bone grafts in rabbits.

Abstract

Event Abstract Back to Event Effect of bone regeneration of CMC- and HA-based bone grafts in rabbits. Soo Jin Son1, Seong Soo Kang2, Jun Sik Son3 and Seok Hwa Choi1* 1 Chungbuk National University, Veterinary Medical Center, Korea 2 Chonnam National University, Department of Veterinary Surgery, Korea 3 Korea Textile Development Institute, Fusion Textile Research, Korea Although autogenous bone is the most widely used graft material for bone defects, demineralized bone matrix materials are available as alternatives to autograft. This study was undertaken to assess the bone regeneration of bone graft substitutes in a rabbit segmental defect model. Comparison of bone regeneration between carboxymethylcellulose (CMC)-based bone source and hyaluronic acid (HA)-based bone source was the primary interest of the study, and to determine whether CMC-based demineralized or non-demineralized bone sources produce bone regeneration in a rabbit segmental defect model. Each 15-mm segmental defect in both left and right radiuses was created in 36 New Zealand White rabbits and filled either with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB) in random order. Additional two rabbits as control were used to no treatment and they were used to radiographic examination only during 12 weeks post-operation. Four rabbits from each group were sacrificed at 4, 8, and 12 weeks post-implantation. The wound area was evaluated by radiology, micro-computerized tomography (CT) and histopathology. DBX has significantly lower radiopacity, bone volume fraction, and bone mineral density compared to DB and NDDB before implantation. However, there were no significant differences in bone healing capacity as revealed by similar bone healing score, bone volume fraction, bone mineral density, and residual bone area in radiographic assessment, micro-CT, and histological evaluation at 4, 8, and 12 weeks post-implantation. In conclusion, application of CMC-based and HA-based bone graft substitutes in a segmental bone defect rabbit model results in similar effects on bone regeneration.