Abstract
ObjectiveTo investigate the molecules related to bone morphogenetic protein (BMP)signaling pathway in adipose-derived stem cells (ADSCs) and the effects of bone defect on their expression in rat endogenous ADSCs.MethodsThirty Wistar rats were randomly divided into a control group (group A, n =5) and an experimental group (group B, n =25). Rats in group A were not treated before their inguinal adipose pads were used to culture ADSCs. Rats in group B were first made into models of bone defect before their inguinal adipose pads were obtained to culture ADSCs in vitro 1, 3, 7, 14 and 21 days after modeling (5 rats for each time). After 7 days of primary culture, the total RNA was extracted from ADSCs to detect the expressions of BMP receptors and Smads by RT-qPCR.ResultsThe expression of BMPR1awas significantly increased in group B at all time points compared with group A ( P ＜ 0. 05 ), and reached the peak at 7 days after bone defect (2. 532,2. 552). The expression of BMPR1b was significantly increased in group B at 1, 3, 7 and 14 days compared with group A ( P ＜ 0. 05), and reached the peak at 14 days after bone defect (6. 628,6. 648). The expressions of BMPR2, Smad5 and Smad8 were significantly increased in group B at 3, 7, 14 and 21 days compared with group A ( P ＜ 0. 05). The expressions of BMPR2 and Smad5 reached the peak at 14 days after bone defect(3. 538, 3. 658; 8. 055, 8. 149), and the expression of Smad8 reached the peak at 7 days (3. 657,3. 759). The expression of Smad1 was significantly increased in group B at 7, 14 and 21 days compared with group A ( P ＜ 0. 05), and reached the peak at 3 days after bone defect (3. 641,3. 771 ).ConclusionsSince ADSCs can express BMP receptors, there may be a BMP signaling pathway in them. A bone defect may possibly induce an increase in expression of the molecules related to BMP signaling pathway in ADSCs. Key words: Adult stem cells; Adipose tissue; Bone morphogenetic protein receptors; Bone defect
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