Effect of BMP-2 on Gene Expression of Enamel Matrix Proteins at the Dental Epithelial Cell Line

Liming Xu,Ryoya Takahashi,Hidemitsu Harada,Akiyoshi Taniguchi

doi:10.2174/1874070700701010018

Abstract

Epithelial-mesenchymal interactions play an important role in the control of ameloblasts and odentoblasts differentiation, and the bone morphogenetic proteins (BMPs) are known factors that regulate the differentiation of ameloblasts. We examined the effect of BMP-2 on the expression of the enamel matrix protein genes at the dental epithelial cell line. BMP-2 induced a 3- to 4-fold increase in amelogenin and ameloblastin mRNA expression, suggesting that BMP-2 is important for ameloblast differentiation. This finding has potential application in the tissue engineering of tooth re-constructions.

Highlights

The interactions between epithelial and mesenchymal cells are thought to play an important role in the control of proliferation and differentiation of these cells
In the present study we examined the effect of bone morphogenetic proteins (BMPs)-2 on the gene expression of the enamel matrix proteins at the dental epithelial cell line
We found that 100 ng/ml of BMP-2 induced up-regulation of endogenous amelogenin and ameloblastin mRNA expression in HAT-7 cells (Fig. 1)

Summary

INTRODUCTION

The interactions between epithelial and mesenchymal cells are thought to play an important role in the control of proliferation and differentiation of these cells. Epithelial-mesenchymal interactions play an important role in the control of ameloblast and odentoblast differentiation. Amelogenin and ameloblastin, which are major proteins of the enamel matrix produced by epithelial ameloblasts, are known to play a role in the regulation of mineralization of enamel [1,2]. We previously demonstrated that the reuptake of full-length amelogenin protein results in increased levels of amelogenin mRNA through enhanced mRNA stabilization [6,7] These findings indicated that amelogenin proteins are important for both ameloblast and odentoblast differentiation in an autocrine or paracrine manner. This finding has potential application in the tissue engineering of tooth re-constructions

MATERIALS AND METHODS

Findings

DISCUSSION