Effect of blue light blocking glass on clock gene expression, melatonin secretion and sleep quality in humans

Abstract

Light has a strong effect on the circadian rhythm. Blue light at night has a negative effect on melatonin secretion and sleep quality. Recently, a smart phone is popularly used in the whole day even including night, resulting in the increased rate of insomnia and difficulties of quality of sleep. In the present study, we investigated that the effect of blue light blocking glass on clock gene expression, melatonin secretion and sleep quality in humans. Seven men participated in this study for overnight from 18:00 h to 8:00 h of next day. Each subject underwent two conditions with and without wearing blue light blocking glass on a different day. Subjects sat in front of 5000 lux fluorescent and blue light lumps until sleep at 0:00 h in a climatic chamber set at 26°C and 50% relative humidity. Subjects were measured Electrocardiogram during the experiment and Electroencephalogram during the night. Saliva samples were collected at 20:00 h, 23:30 h, 6:00 h and 8:00 h. Total RNA was isolated from saliva sample and was measured the clock gene expressions of Clock, Bmal1, Per1 and Cry1 by real‐time PCR. Clock gene expression were not significantly different between the two groups. The melatonin concentrations were not significantly different between the two groups. Total percentage of slow‐wave sleep and the sleep latency to stage 3 were not significantly different with both conditions. Our results suggested that blue blocking glass have individual effects under strong light by 5000 lux.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.