Abstract
Objective: To detect the expression of platclet associated immunoglobulin (PAIg) on the ITP patients’ platelet surface and study the effect and correlation of blood transfusion therapy on PAIg. Methods: The 173 patients confirmed as ITP selected form the outpatients and inpatients of the Department of Hematology and Rheumatology in the Third Affiliated Hospital of Guizhou Medical University, were divided into the blood transfusion group and the non-blood transfusion group, FCM fluorescence immunolabeling was used to measure expression quantity of PAIg, the positive expression rate and fluorescence intensity of PAIgG, PAIgM and PAIgA expressed on the platelet surface of ITP patients and the control group respectively. The softwave SPSS12.0 was applied to analyze the correlation. Results: (1) PAIgG (2.96 + 8.31) %, PAIgM (2.27 + 1.78) %, PAIgA (4.48 + 3.88) % in the non-transfusion group were significantly lower than those in the transfusion group (11.44 + 20.04) %, (9.72 + 15.24) %, (10.13 + 9.53) %, P 0.05. Conclusion: (1) It can increase platelet antibody after blood transfusion, and patients with ITP are more likely to produce platelet antibody after blood transfusion. (2) There is a certain degree of correlation between PAIg and blood transfusion in ITP patients.
Highlights
Idiopathic thrombocytopical purpura (ITP) is a clinically common hemorrhagic disease that destroys platelets by generating autoantibodies [1]
(2) There is a certain degree of correlation between platclet associated immunoglobulin (PAIg) and blood transfusion in ITP patients
We examined the changes of platelet-associated antibodies in 173 patients with and without ITP transfusions, and 20 healthy subjects were selected as the control group to explore the influence of blood transfusion on platelet antibodies in ITP patients
Summary
Idiopathic thrombocytopical purpura (ITP) is a clinically common hemorrhagic disease that destroys platelets by generating autoantibodies [1]. Megakaryocytes cause serious damage to the compounds and reduce the number of platelets, as in platelet membrane glycoproteins (glycoprotein, GP) IIb/IIIa, Ib/IX, Ia/IIa, immunoglobulin (platelet associated immunity globin, PAIg) antibodies, after immunoglobulin antibodies combined with platelets and adsorbed on the surface of platelet antigen promoting the mononuclear macrophage system of platelet phagocytosis and clear [5]. Changes in platelet autoantibody levels provide important information in assessing patient responses to treatments and disease prognosis. PAIG is a kind of autoantibodies that produced in pathological immune responses to platelet membrane glycoprotein GPII/IIIa and bIb/IX antigens. These antibodies bind to their corresponding antigens on the platelet membrane, subsequently, platelets are largely destroyed by the mononuclear - phagocytic system. We examined the changes of platelet-associated antibodies in 173 patients with and without ITP transfusions, and 20 healthy subjects were selected as the control group to explore the influence of blood transfusion on platelet antibodies in ITP patients
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