Effect of blood contamination on the push-out bond strength of four endodontic root perforation repair materials: An in vitro study

Abstract

Aim: To evaluate the push-out bond strength of four endodontic root perforation repair materials on blood contamination. Materials and Methods: Freshly extracted 80 single-rooted human canine teeth were used. The crowns of all teeth were removed, and the mid-root dentin was sectioned horizontally into slices with a thickness of 1.0mm by using a diamond disk. In 40 samples, a 27-gauge syringe was used to inject blood into the perforated area, which was taken from the patient. Biodentine (Septodont, Pennsylvania, USA) liquid from a single dose container was emptied into a powder-containing capsule and mixed 30 seconds, was mixed in 10 samples; glass ionomer cement (GIC) (GC Fuji IX; GC, Tokyo, Japan) was mixed in 10 samples; ProRoot mineral trioxide aggregate (MTA) (DENTSPLY, Tulsa Dental Specialties, Tulsa, Oklahoma), hand mixed with sterile water at a powder to liquid ratio of 3:1 in accordance with the manufacturer’s instructions, was mixed in 10 samples; and Cavit (ESPE Dental Products, Ohio, USA) was mixed in 10 samples and placed in perforation area. In the remaining 40 samples, normal saline irrigation was performed before restoring 10 samples with Biodentine, 10 samples with GIC, 10 samples with ProRoot MTA, and 10 samples with Cavit. The samples were carried in wet gauze piece and placed in a closed container in an incubator at 370°C and 100% humidity until push-out bond strength was carried out. Results: The result was statistically analyzed using one-way analysis of variance (ANOVA) and Tukey’s post hoc tests. P <0.05 was considered as significant. Conclusion: Push-out bond strength of Biodentine was higher than that of ProRoot MTA, GIC, and Cavit on blood contamination.