Effect of Blood and Synthetic Tissue Fluid on Marginal Adaptation and Surface Microstructure of Different Root-end Filling Materials: A Scanning Electron Microscope Study

Abstract

Aim: This study aimed to evaluate the effect of blood and synthetic tissue fluid on marginal adaptation and surface microstructure of Mineral trioxide aggregate (MTA) and Endosequence root repair material (ERRM Putty).
 Materials and Methods: Sixty human single-rooted teeth were endodontically treated, and root-end cavities were prepared to 3 mm depth. The teeth were divided randomly into three groups (n = 20) based on the contamination, and each group was again divided into two subgroups (n=10) based on the root-end filling material used.
 Group I (Blood Exposed): The root-end cavities were filled with fresh blood then gently aspirated. The retro cavities were then filled with MTA and ERRM putty and placed in molds containing heparinized blood.
 Group II (STF-Exposed): STF was mixed accordingly and the retro cavities were then filled with STF and then gently aspirated. The cavities were now filled with MTA and ERRM putty and placed in molds containing STF.
 Group III (Control): The root end cavities were not contaminated with any fluid and were filled with MTA and ERRM putty in this group. All samples were viewed Under SEM at 2000x magnification and statistically analyzed with Tukey post hoc test.
 Results: Mean gap width in microns (SEM) was blood with MTA(22.12), followed by blood with ERRM putty (18.89), Control with ERRM putty (14.53), STF with MTA (6.86), Control with MTA (6.85) and STF with ERRM putty (3.78). A significant difference was observed among the three groups (Blood, STF and Control) with gap width in microns (SEM) at 5% level of significance. 
 Conclusions: The samples contaminated with blood showed more gap width, and ERRM putty exhibited better marginal adaptation.