Effect of Bletilla striata on the Prevention of Postoperative Peritoneal Adhesions in Abrasion-Induced Rat Model.

Botong Liu,Jian Wang,Yunxia Guan,Yilan Liu,Hui Wang,Qiuhua Zhang,Xiao Wu,Yanjun Fu,Wencong Cao,Shicheng Yang

doi:10.1155/2019/9148754

Abstract

Postoperative peritoneal adhesions (PPAs) constitute a common complication of abdominal surgery with a high incidence. Bletilla striata (BS) is an important hemostatic drug used in China for nearly 2000 years. The purpose of this study was to investigate the effect of Bletilla striata on postoperative intestinal adhesion in rats. PPA was induced by cecal wall abrasion, and Bletilla striata was injected to observe its effect on adhesion in rats. The adhesion and inflammation score were assessed through visual observation and histopathologic evaluation. The levels of interleukin-1 (IL-1β), tumor necrosis factor (TNF-α), and interleukin-17F (IL-17F) in abdominal cavity and interleukin-6 (IL-6) in plasma were measured by enzyme-linked immunosorbent assay (ELISA) at 6 hours, 12 hours, 24 hours, and 1 week after operation. The tissue level of transforming growth factor beta-1 (TGF-β1) was also determined by ELISA on the seventh day after surgery. The expressions of collagen and TNF-α were, respectively, detected by Masson trichrome staining and immunohistochemical staining. The expression of TGF-β1 and alpha smooth muscle actin (α-SMA) was detected by Western blot. The result showed that Bletilla striata has obvious preventive effect on PPAs and celiac inflammation of PPAs. Bletilla striata could significantly reduce the level of IL-17F abdominal cavity and IL-6 in plasma. Masson trichrome staining and immunohistochemical staining results showed that Bletilla striata also decreased the expression of TNF-α and collagen. Western blot results showed that Bletilla striata decreased the expression of α-SMA and TGF-β1. Our results suggest that Bletilla striata decreased the development of abdominal adhesion in abrasion-induced model of rats and reduced the expression of the important substance which increased in PPAs. Bletilla striata can be further studied as a new and cheaper antiadhesive substance.

Highlights

Postoperative peritoneal adhesions (PPAs) constitute a common complication of abdominal surgery; the incidence rate is up to 90%-95% [1]
A research confirmed its protective effect on renal fibrosis, which is probably mediated by downregulated alpha smooth muscle actin (α-SMA) in vitro. [8]; we speculate that Bletilla striata can prevent abdominal adhesion after operation
The current study found that Bletilla striata, a widely used astringent hemostatic medicinal plant, can effectively prevent PPAs by observing the degree of abdominal adhesion on the 7th day after operation

Summary

Introduction

Postoperative peritoneal adhesions (PPAs) constitute a common complication of abdominal surgery; the incidence rate is up to 90%-95% [1]. PPAs may cause significant morbidity, including adhesive small bowel obstruction, female infertility, chronic abdominal pain, and increased difficulty with subsequent surgery [2, 3]. Preventive measures include keeping the lesions separated, increasing bowel motility, and decreasing local inflammation [6]. There is no widely accepted method of treating abdominal adhesions, so we still need to find a more effective treatment method to prevent abdominal adhesion after operation. (Orchidaceae) is an important astringent hemostatic medicinal plant used for almost 2000 years in China and is widely distributed in eastern and southern Asia. [8]; we speculate that Bletilla striata can prevent abdominal adhesion after operation A research confirmed its protective effect on renal fibrosis, which is probably mediated by downregulated alpha smooth muscle actin (α-SMA) in vitro. [8]; we speculate that Bletilla striata can prevent abdominal adhesion after operation

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