Effect of Black Tea Polyphenol on Cell-ECM Interaction and MMP

Nabanita Bhattacharyya,Shuvojit Moulik,Amitava Chatterjee,Md Nasim Ali,Subhajit Mondal,Shreoshi Bhattacharrya,Alok Kumar Hazra,Sathi Paul,Anjan Adhikari

doi:10.4236/ajps.2017.84058

Abstract

Tea is one of the most widely consumed beverages in the world. Black tea, obtained from the leaves of Camellia sinensis is the preferred beverage in India and in most western countries. Epidemiological studies on black tea and cancer are limited. However, preliminary studies indicate a positive correlation between black tea consumption and a lower incidence of breast and ovarian cancer. In the present communication, we wanted to see the effect of black tea extract and the polyphenol theaflavin on cell-ECM interaction, MMP activity etc. to strengthen the anti-cancer effect of black tea.

Highlights

Tea is one of the most widely consumed beverages in the world
After 24 and 48 hrs of treatment in case of black tea extract the activated 68 kD band was absent within 48 hrs but in case of theaflavin treated cells both the pro and activated bands of Matrix metalloproteinases (MMPs)-2 were absent within 48 hrs of treatment
The results clearly show that the binding of black tea extract and theaflavin treated B16F10 cells reduced drastically to fibronectin (ECM) most probably via integrin alfa5beat1

Summary

Introduction

Tea is one of the most widely consumed beverages in the world. Black tea, obtained from the leaves of Camellia sinensis is the preferred beverage in India and in most western countries [1] [2]. Theaflavins are anti-oxidant polyphenols formed via co-oxidation of pairs of epimerized catechins during fermentation of tea leaves in black tea and account for 2% - 6% of dry weight. Theaflavins are characterized by benzotropolone ring structure and contribute to the unique taste of black tea. Theaflavins are the major antioxidant constituents of black tea, inhibiting free radical generation and pro-oxidative enzyme activities and chelating metal ions to prevent lipid peroxidation. TF-3 has been shown to possess a higher antioxidative activity than catechins, including epigallocatechin gallate (EGCG) [1] [2] [4]

Methods

Results

Conclusion