Effect of Black Tea Extract on Hypoglycemia Induced by Plasmodium berghei ANKA Infection in Mice

Muthita Sihabud,Puangphaka Yongthawee

doi:10.4172/2470-6965.1000s1-005

Abstract

Hypoglycemia was found as one of all causes of death in malaria disease and urgently needed to fine new drugs to treat this condition. Hence, the present study was aimed to evaluate the effect of black tea extract on hypoglycemia induced by Plasmodium berghei infection in mice. Aqueous crude extract of black tea was freshly prepared using hot water method and used for efficacy test in vivo. For in vivo test, P. berghei ANKA infected mice were given orally by gavage with 500, 1000, and 2000 mg/kg of black tea extract for 4 consecutive days. Blood glucose levels were then measured. It was found that aqueous crude extract of black tea exerted dose-dependent anti-hypoglycemic activity, especially at a dose of 2000 mg/kg showed the highest activity. Although, pyrimethamine treated group showed significantly (p<0.01) decreasing of blood glucose levels, combination treatment with black tea extract could protect and maintain blood glucose to normal level. It can be concluded that aqueous crude extract of black tea presented anti-hypoglycemic activity against P. berghei infection in mice.

Highlights

Malaria remains a major public health problem in tropical and sub-tropical areas such as Africa, North and South America, Asia and Southeast Asia including Thailand
This could be due to the fact that during malaria infection and development in blood stage, glucose is rapidly taken up across the parasite plasma membrane through a facilitated hexose transporter and is in turn metabolized through the process of glycolysis for energy supply [12]
It has been described that it is accompanied with approximately 100-fold increase in glucose utilization when compared to uninfected red blood cells so causing a profound hypoglycemia if untreated [13]

Summary

Introduction

Malaria remains a major public health problem in tropical and sub-tropical areas such as Africa, North and South America, Asia and Southeast Asia including Thailand. Malariaassociated hypoglycemia is one of all causes of death in P. falciparum and P. vivax severe malaria. Activation of glucose uptake into infected red blood cells, oxidative stress, and hemolysis has been described to cause hypoglycemia during malaria infection [4]. This has prompted research towards the discovery of new drugs with anti-hypoglycemic property. Black tea extract in malaria researches and hypoglycemia protection during malaria infection have not yet been studied. He once, this study was aimed to investigate antihypoglycemic effect of black tea extract against P. berghei infected mice

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Results

Conclusion